rbms3 antibody  (Bio-Techne corporation)

Journal: Heliyon

Article Title: Tumor suppressor function of RBMS3 overexpression in EOC associated with immune cell infiltration

doi: 10.1016/j.heliyon.2024.e30603

Figure Lengend Snippet: Primer sequences.

Article Snippet: Prior to being incubated with primary antibodies against RBMS3 (1:2000; No.: DF8599; Affinity Biologicals, China) or GAPDH (1:10,000; No.: AF7021; Affinity Biologicals, China) for an entire night on a shaking table at 4 °C, for 2 h under a condition of the room temperature, 5% skim milk was applied to block the membranes.

Techniques:

Journal: Heliyon

Article Title: Tumor suppressor function of RBMS3 overexpression in EOC associated with immune cell infiltration

doi: 10.1016/j.heliyon.2024.e30603

Figure Lengend Snippet: Antibody information used in the experiment.

Article Snippet: Prior to being incubated with primary antibodies against RBMS3 (1:2000; No.: DF8599; Affinity Biologicals, China) or GAPDH (1:10,000; No.: AF7021; Affinity Biologicals, China) for an entire night on a shaking table at 4 °C, for 2 h under a condition of the room temperature, 5% skim milk was applied to block the membranes.

Techniques:

Journal: Heliyon

Article Title: Tumor suppressor function of RBMS3 overexpression in EOC associated with immune cell infiltration

doi: 10.1016/j.heliyon.2024.e30603

Figure Lengend Snippet: The poor prognosis of OC patients is linked to down-regulated RBMS3 expression in OC tissues. (A) RBMS3 mRNA expression levels from the GEPIA database in both normal tissue and OC. Grey (n = 88) normal samples and red (n = 426) tumor samples. (B, C)Data on OS and PFS for patients with OC were collected with the assistant of the Kaplan-Meier plotter ( https://kmplot.com/analysis/ ). (D) qRT-PCR was used to measure the mRNA levels of RBMS3 in 29 instances of EOC and 23 cases of normal ovarian tissue. (E, F) Western blot analysis reveals the RBMS3 protein expression level in eight pairs of EOC tissues. (G) Typical IHC staining pictures of RBMS3 in normal tissue (Normal) and OC (Tumor). The scale bars are 50 μm and 20 μm respectively. (H, I) Kaplan-Meier study of the effects of 60 EOC patients' RBMS3 expression on OS and PFS. * P < 0.05, ** P < 0.01, *** P < 0.001 and**** P < 0.0001.The uncropped versions of E is in .

Article Snippet: Prior to being incubated with primary antibodies against RBMS3 (1:2000; No.: DF8599; Affinity Biologicals, China) or GAPDH (1:10,000; No.: AF7021; Affinity Biologicals, China) for an entire night on a shaking table at 4 °C, for 2 h under a condition of the room temperature, 5% skim milk was applied to block the membranes.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Immunohistochemistry

Journal: Heliyon

Article Title: Tumor suppressor function of RBMS3 overexpression in EOC associated with immune cell infiltration

doi: 10.1016/j.heliyon.2024.e30603

Figure Lengend Snippet: Expression of RBMS3 in EOC and normal tissue.

Article Snippet: Prior to being incubated with primary antibodies against RBMS3 (1:2000; No.: DF8599; Affinity Biologicals, China) or GAPDH (1:10,000; No.: AF7021; Affinity Biologicals, China) for an entire night on a shaking table at 4 °C, for 2 h under a condition of the room temperature, 5% skim milk was applied to block the membranes.

Techniques: Expressing

Journal: Heliyon

Article Title: Tumor suppressor function of RBMS3 overexpression in EOC associated with immune cell infiltration

doi: 10.1016/j.heliyon.2024.e30603

Figure Lengend Snippet: The relation between clinical parameters and the expression of RBMS3.

Article Snippet: Prior to being incubated with primary antibodies against RBMS3 (1:2000; No.: DF8599; Affinity Biologicals, China) or GAPDH (1:10,000; No.: AF7021; Affinity Biologicals, China) for an entire night on a shaking table at 4 °C, for 2 h under a condition of the room temperature, 5% skim milk was applied to block the membranes.

Techniques: Expressing

Journal: Heliyon

Article Title: Tumor suppressor function of RBMS3 overexpression in EOC associated with immune cell infiltration

doi: 10.1016/j.heliyon.2024.e30603

Figure Lengend Snippet: The overexpression and suppression of the RBMS3 gene can control the capabilities to migrate, invade, and reproduce of OC cells. (A, B) Stable ID8 cell lines that overexpress and knockdown RBMS3 were created using the lentiviral transfection approach. The fluorescence plot illustrates representative images of stably transfected cells in both dark and bright settings (Microscopic magnification: 20 × ). (C) Comparative mRNA expression levels of RBMS3 in lentiviral-transfected ID8 cells. (D) Western Blot analysis verified that ID8 cells had overexpressed and knocked down RBMS3 genes. (E, F) Plate cloning and CCK-8 were used to determine cell growth. (G, H) Experiments on wound healing demonstrate how RBMS3 affects the capacity for cell migration (Microscopic magnification: 4 × ). (I, J) The Transwell technique for identifying the capacity for the capabilities in invading and migrating of cells (Microscopic magnification: 10 × ). The scale bar is 100 μm * P < 0.05, ** P < 0.01, and *** P < 0.001. The uncropped versions of D is in .

Article Snippet: Prior to being incubated with primary antibodies against RBMS3 (1:2000; No.: DF8599; Affinity Biologicals, China) or GAPDH (1:10,000; No.: AF7021; Affinity Biologicals, China) for an entire night on a shaking table at 4 °C, for 2 h under a condition of the room temperature, 5% skim milk was applied to block the membranes.

Techniques: Over Expression, Control, Knockdown, Transfection, Fluorescence, Stable Transfection, Expressing, Western Blot, Cloning, CCK-8 Assay, Migration

Journal: Heliyon

Article Title: Tumor suppressor function of RBMS3 overexpression in EOC associated with immune cell infiltration

doi: 10.1016/j.heliyon.2024.e30603

Figure Lengend Snippet: The RBMS3 gene induces cell cycle arrest and apoptosis, potentially preventing the progression of OC cells. (A, B) Histograms of the flow cytometry data and flow cytometry detection of the cell cycle distribution of ID8 cells before and after RBMS3 gene overexpression and knockdown. (C, D) Using flow cytometry, the apoptotic rate of ID8 cells with RBMS3 lentivirus overexpression and knockdown was examined. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Prior to being incubated with primary antibodies against RBMS3 (1:2000; No.: DF8599; Affinity Biologicals, China) or GAPDH (1:10,000; No.: AF7021; Affinity Biologicals, China) for an entire night on a shaking table at 4 °C, for 2 h under a condition of the room temperature, 5% skim milk was applied to block the membranes.

Techniques: Flow Cytometry, Over Expression, Knockdown

Journal: Heliyon

Article Title: Tumor suppressor function of RBMS3 overexpression in EOC associated with immune cell infiltration

doi: 10.1016/j.heliyon.2024.e30603

Figure Lengend Snippet: Immune cell infiltration and RBMS3 expression are correlated in OC tissues. (A) TISIDB database data shows the connection between RBMS3 expression and 28 tumor-infiltrating immune cells in human cancer (red indicates positive infiltration, blue indicates negative). (B) The degree of immune cell infiltration in OC tissues is correlated with the expression of RBMS3. (C) An IHC staining diagram showing the relationship between immune cell-related markers and RBMS3 expression (magnification: 400 × ). The scale bar is 20 μm.

Article Snippet: Prior to being incubated with primary antibodies against RBMS3 (1:2000; No.: DF8599; Affinity Biologicals, China) or GAPDH (1:10,000; No.: AF7021; Affinity Biologicals, China) for an entire night on a shaking table at 4 °C, for 2 h under a condition of the room temperature, 5% skim milk was applied to block the membranes.

Techniques: Expressing, Immunohistochemistry

Journal: Heliyon

Article Title: Tumor suppressor function of RBMS3 overexpression in EOC associated with immune cell infiltration

doi: 10.1016/j.heliyon.2024.e30603

Figure Lengend Snippet: The relationship between high and low RBMS3 expression in ID8 cells and immune cell markers. (A,B) RBMS3 expression in ID8 cells in both groups. (C,D) Immunofluorescence imagesof INOS, ARG1, FOXP3 and CD11b in cells of Lv-Vector and Lv-RBMS3 groups (200 × and 400 × ). The scale bars are 100 μm and 50 μm respectively.

Article Snippet: Prior to being incubated with primary antibodies against RBMS3 (1:2000; No.: DF8599; Affinity Biologicals, China) or GAPDH (1:10,000; No.: AF7021; Affinity Biologicals, China) for an entire night on a shaking table at 4 °C, for 2 h under a condition of the room temperature, 5% skim milk was applied to block the membranes.

Techniques: Expressing, Immunofluorescence, Plasmid Preparation

Journal: Heliyon

Article Title: Tumor suppressor function of RBMS3 overexpression in EOC associated with immune cell infiltration

doi: 10.1016/j.heliyon.2024.e30603

Figure Lengend Snippet: Impact of overexpressing the RBMS3 gene on in vivo angiogenesis and tumor growth. (A) Diagram of mouse subcutaneous tumor growth. (B) Tumor weight 28 days after inoculating cells. (C) Mouse weight growth curve. (D) Measure the subcutaneous tumor volume, tumor volume = 0.5 × largest dimension × shortest dimension 2 . (E, F) Immunohistochemistry (IHC) staining of Ki-67 and CD31 in transplanted tumor tissue Lv-Vector and Lv-RBMS3 groups (200 × and 400 × ). The scale bars are 50 μm and 20 μm respectively.

Article Snippet: Prior to being incubated with primary antibodies against RBMS3 (1:2000; No.: DF8599; Affinity Biologicals, China) or GAPDH (1:10,000; No.: AF7021; Affinity Biologicals, China) for an entire night on a shaking table at 4 °C, for 2 h under a condition of the room temperature, 5% skim milk was applied to block the membranes.

Techniques: In Vivo, Immunohistochemistry, Plasmid Preparation

Journal: Heliyon

Article Title: Tumor suppressor function of RBMS3 overexpression in EOC associated with immune cell infiltration

doi: 10.1016/j.heliyon.2024.e30603

Figure Lengend Snippet: The relationship between high and low expression of RBMS3 in tumor tissues and immune cell markers. (A,B) IHC staining detects the expression of RBMS3 in mouse tumor tissues. (C,D) Immunofluorescence detection images of INOS, ARG1, FOXP3 and CD11b in the Lv-Vector and Lv-RBMS3 groups of transplanted tumor tissues (200 × and 400 × ). The scale bars are 50 μm and 20 μm respectively.

Article Snippet: Prior to being incubated with primary antibodies against RBMS3 (1:2000; No.: DF8599; Affinity Biologicals, China) or GAPDH (1:10,000; No.: AF7021; Affinity Biologicals, China) for an entire night on a shaking table at 4 °C, for 2 h under a condition of the room temperature, 5% skim milk was applied to block the membranes.

Techniques: Expressing, Immunohistochemistry, Immunofluorescence, Plasmid Preparation

Journal: Heliyon

Article Title: Tumor suppressor function of RBMS3 overexpression in EOC associated with immune cell infiltration

doi: 10.1016/j.heliyon.2024.e30603

Figure Lengend Snippet: Effects of RBMS3 on the Wnt/β-catenin signalling pathway in ovarian cancer cells. (A, B) Up-regulation of RBMS3 regulates the Wnt/β-catenin signalling pathway in ID8 cells. (C) After overexpression of RBMS3 and activation of the Wnt/β-catenin signalling pathway, the migratory activity of ID8 cells was detected by transwell. (D) After overexpression of RBMS3 and activation of the Wnt/β-catenin signalling pathway, the invasive activity of ID8 cells was detected by transwell. The scale bars is 100 μm ** P < 0.01, *** P < 0.001 and **** P < 0.0001. The uncropped versions of A is in .

Article Snippet: Prior to being incubated with primary antibodies against RBMS3 (1:2000; No.: DF8599; Affinity Biologicals, China) or GAPDH (1:10,000; No.: AF7021; Affinity Biologicals, China) for an entire night on a shaking table at 4 °C, for 2 h under a condition of the room temperature, 5% skim milk was applied to block the membranes.

Techniques: Over Expression, Activation Assay, Activity Assay

Journal: Technology in Cancer Research & Treatment

Article Title: Tumor Suppressor Effect of RBMS3 in Breast Cancer

doi: 10.1177/15330338211004921

Figure Lengend Snippet: Clinical and Pathological Characteristics of 127 Patients With Breast Cancer.

Article Snippet: Next, the tissues were incubated with rabbit anti-RBMS3 antibody (1:100, Novus, USA) overnight at 4°C, followed by incubation with horseradish peroxidase–linked secondary antibody for 30 min at room temperature.

Techniques:

Journal: Technology in Cancer Research & Treatment

Article Title: Tumor Suppressor Effect of RBMS3 in Breast Cancer

doi: 10.1177/15330338211004921

Figure Lengend Snippet: RBMS3 expression in breast cancer tissues (T) and adjacent non-tumorous (N) tissues from The Cancer Genome Atlas (TCGA) database, and the relations between RBMS3 expression and overall survival (OS) rate. A) RBMS3 mRNA expression in 1,092 breast cancer tissue specimens was lower than that in 111 NAT ( P < 0.001). The red lines represent the mean and standard deviation. B) RBMS3 mRNA expression was lower in 111 breast cancer tissue specimens than in paired NAT ( P < 0.001). C) Kaplan–Meier survival curve showing a higher OS rate in the RBMS3 high expression group as compared to the RBMS3 low expression group ( P = 0.004).

Article Snippet: Next, the tissues were incubated with rabbit anti-RBMS3 antibody (1:100, Novus, USA) overnight at 4°C, followed by incubation with horseradish peroxidase–linked secondary antibody for 30 min at room temperature.

Techniques: Expressing, Standard Deviation

Journal: Technology in Cancer Research & Treatment

Article Title: Tumor Suppressor Effect of RBMS3 in Breast Cancer

doi: 10.1177/15330338211004921

Figure Lengend Snippet: RBMS3 expression in breast cancer tissue and NAT. A) qRT-PCR showing lower RBMS3 mRNA expression in breast cancer tissues compared to NAT (* P < 0.05). RBMS3 expression was normalized to the internal control GAPDH. B) Representative immunohistochemical (IHC) staining images of RBMS3 expression and location (brown nuclear staining) in breast cancer tissue and normal breast tissue specimens (×200 magnification).

Article Snippet: Next, the tissues were incubated with rabbit anti-RBMS3 antibody (1:100, Novus, USA) overnight at 4°C, followed by incubation with horseradish peroxidase–linked secondary antibody for 30 min at room temperature.

Techniques: Expressing, Quantitative RT-PCR, Control, Immunohistochemical staining, Immunohistochemistry, Staining

Journal: Technology in Cancer Research & Treatment

Article Title: Tumor Suppressor Effect of RBMS3 in Breast Cancer

doi: 10.1177/15330338211004921

Figure Lengend Snippet: Relations Between RBMS3 Expression Level and Clinical and Pathological Data.

Article Snippet: Next, the tissues were incubated with rabbit anti-RBMS3 antibody (1:100, Novus, USA) overnight at 4°C, followed by incubation with horseradish peroxidase–linked secondary antibody for 30 min at room temperature.

Techniques: Expressing

Journal: Technology in Cancer Research & Treatment

Article Title: Tumor Suppressor Effect of RBMS3 in Breast Cancer

doi: 10.1177/15330338211004921

Figure Lengend Snippet: Kaplan–Meier analysis of overall survival (OS) in patients with breast cancer based on RBMS3 and cofactor expression. A) OS rate of RBMS3 high versus RBMS3 low expression in all patients ( P = 0.017). B) OS rate of patients with American Joint Committee on Cancer (AJCC) stage I/II disease ( P = 0.035). C) OS rate of patients with T1/T2 disease ( P = 0.018). D) OS rate of patients with N0/N1 disease ( P = 0.014). E) OS rate of cofactor_high versus cofactor_low expression in all patients ( P = 0.001).

Article Snippet: Next, the tissues were incubated with rabbit anti-RBMS3 antibody (1:100, Novus, USA) overnight at 4°C, followed by incubation with horseradish peroxidase–linked secondary antibody for 30 min at room temperature.

Techniques: Expressing

Journal: Technology in Cancer Research & Treatment

Article Title: Tumor Suppressor Effect of RBMS3 in Breast Cancer

doi: 10.1177/15330338211004921

Figure Lengend Snippet: Univariate and Multivariate Analysis of Variables and Breast Cancer Prognosis.

Article Snippet: Next, the tissues were incubated with rabbit anti-RBMS3 antibody (1:100, Novus, USA) overnight at 4°C, followed by incubation with horseradish peroxidase–linked secondary antibody for 30 min at room temperature.

Techniques: Expressing